Course details
The 1st week of the Animal Cell Culture microcredential introduces you to the animal cell culture lab and operation of equipment, including biological safety cabinets and the biosafety regulations for working with different types of cells. You will immediately begin sub-culturing both adherent and suspension cells with an emphasis on good aseptic technique. You will also learn how to count cells to determine cell density and viability, thaw and cryo-preserve cell stocks and perform a gene transfer (transfection) experiment. Classroom time (~5 hours in-person or online) will be scheduled in between lab activities (~25 hours) to review procedures and build your knowledge base. Topics will include: the various types of animal cells, growth media and supplements used in research, biopharmaceutical and biomanufacturing applications; how to prevent and detect microbial contamination (e.g. mycoplasma testing); gene transfer and analysis techniques.
Prerequisite(s)
- No prerequisites are required for this course.
Credits
2.5
- Not offered this term
- This course is not offered this term. Please check back next term or subscribe to receive notifications of future course offerings and other opportunities to learn more about this course and related programs.
Learning Outcomes
Upon successful completion of this course, the trainee will be able to:
- Explain the different types of cells that are used in animal cell culture, the different types of media and supplements that are necessary for cell growth, and the biosafety regulations for working with animal cells.
- Understand the functions of the different types of equipment found in a cell culture lab.
- Understand the importance of aseptic technique for working with animal cells and the methods used to monitor for microbial contamination.
- Understand the different gene transfer techniques that are typically used for animal cells.
- Culture animal cell lines in a biosafety cabinet/laminar flow hood using good aseptic technique and following all biosafety regulations.
- Perform cell counts to determine the cell density and viability of animal cell cultures.
- Perform cryopreservation (freezing) and thawing of animal cells.
- Perform transfection (gene transfer) of animal cells and assess transfection efficiency using several different methods, including fluorescence-based measurements.
Effective as of Spring/Summer 2022
Related Programs
Animal Cell Culture 1 (XBIO 4010) is offered as a part of the following programs:
School of Health Sciences
- Animal Cell Culture
Microcredential Part-time
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