Course Overview
A comprehensive continuation to the training in diagnostic molecular techniques begun in CLGT 5506. In-class lectures, online resources and hands-on laboratory components of this course will focus on advanced techniques such as Next Generation sequencing, real time PCR, RNA based diagnostic assays and Multiplex Ligation Dependant Probe amplification (MLPA). Students will be trained in speciality software for the analysis, interpretation and validation of these assays. Analysis of data from Next Generation Sequencing (NGS) assays as a method for detection of pathogenic mutations will also be introduced.
Prerequisite(s)
- 50% in CLGT 5506
Credits
7.0
- Not offered this term
- This course is not offered this term. Notify me to receive email notifications when the course opens for registration next term.
Learning Outcomes
By the end of this course, learners will be able to:
- Communicate effectively with the instructor and other students.
- Behave in a professional manner in lecture and lab sessions.
- Prepare specimens for analysis by isolating RNA.
- Use techniques such as real time PCR amplification, reverse transcriptase PCR amplification, MLPA, and capillary electrophoresis to diagnose normal, affected, and carrier status in terms of genetic disease.
- Performs Next Generation sequencing under instructor supervision
- Analyze data from Next Generation sequencing reactions.
- Explain why and when different PCR techniques are used in clinical laboratories.
- Explain how molecular technology can be used to diagnose genetic diseases.
- Adapts effectively to a changing work environment and troubleshoots to solve problems in the molecular laboratory.
- Perform and document lab procedures, observations, technical information, results and quality control on reagents and media.
- Describe emergent molecular diagnostic concepts for the constitutional and cancer lab, such as molecular barcodes, digital droplet PCR and cell free DNA.
- Work safely in the laboratory while performing molecular diagnostic assays.
Effective as of Winter 2023
Related Programs
Molecular Technology 2 (CLGT 6606) is offered as a part of the following programs:
School of Health Sciences
- Clinical Genetics Technology
Advanced Diploma Full-time
Programs and courses are subject to change without notice. Find out more about BCIT course cancellations.