Course Overview
This course builds on nucleic acid testing (NAT) fundamentals from MLSC 1314 & MLSC 2314 and applies them to clinical molecular analysis of human genomic sequences. The goal of this course is to apply molecular diagnostic principles to identify nucleic acid sequences, and assess results, identify sources of interference/error, initiate corrective action and/or follow up testing. NAT technologies discussed in detail include singleplex and multiplex amplification by real-time PCR using dye and probe detection chemistries. Human cellular processes of transcription and translation will be built upon, basic human genetics, and standard nomenclature describing genomic DNA sequence variants will be overviewed. The molecular basis of select disease phenotypes will be discussed, with focus on the advantages and limitations of NAT relative to phenotypic testing. Select other molecular testing methodologies used in the clinical lab to identify DNA polymorphisms and mutations will be discussed.
Prerequisite(s)
- All Level 3 courses and MSLC 2314
Credits
2.5
- Not offered this term
- This course is not offered this term. Notify me to receive email notifications when the course opens for registration next term.
Learning Outcomes
Upon successful completion of this course, the student will be able to:
- Prepare patient DNA samples from clinical specimens using solid-phase spin column, magnetic bead and Chelex isolation/purification techniques
- Handle and store clinical specimens, patient and reagent DNA/RNA, and analytical enzymes following established nucleic acid testing (NAT) protocols
- Evaluate DNA samples using methods such as fluorometry, and spectrophotometry
- Program and operate a thermocycler
- Perform real-time PCR amplification assays to identify:
- p.C282Y and p.H63D mutations of the HFE gene associated with Type 1 Hereditary Hemochromatosis.
- Factor V Leiden c.1691G>A mutation of the FV gene associated with Hereditary Thrombosis
- Perform PCR amplification following established quality assurance and quality control principles and procedures to minimize risk/identify presence of DNA contamination and PCR inhibitors
- Apply molecular principles to assess and determine appropriate PCR amplification conditions for conventional and real time assays
- Identify suspect patient and control PCR results and take appropriate follow-up action as required
- Explain the principles of/interpret results for NAT assays that identify: Factor V Leiden and Prothrombin 20210G>A mutations associated with Hereditary Thrombosis (PCR).
- Reports results that meet quality control criteria and identifies unexpected or implausible results and takes appropriate action prior to reporting
- Documents results accurately
- Practices effective verbal, non-verbal and written communication, active listening, and use of technology appropriately to facilitate communication
- Use appropriate genetic terminology and DNA sequence variant nomenclature to describe patient genotypes.
- Apply the principles of routine practice, utilize personal protective equipment correctly and minimize possible dangers from biological specimens, laboratory supplies and equipment
- Use disinfection and sterilization methods and minimize potential hazards related to disinfection/sterilization methods
- Apply spill containment and clean up procedures for biological and other hazardous materials
- Apply proper ergonomic principles to minimize risk of injury & contamination of work
Effective as of Winter 2026
Related Programs
Molecular Diagnostics 2 (MLSC 3314) is offered as a part of the following programs:
School of Health Sciences
- Medical Laboratory Science
Diploma Full-time
Programs and courses are subject to change without notice. Find out more about BCIT course cancellations.