Forensic Biology: DNA Typing Applications FSCT 8160

Learning Outcomes

Upon successful compleltion of this course, the student will be able to:

• Execute biological screening of forensic evidence utilizing presumptive and confirmatory tests for various body fluids.
• Demonstrate critical thinking skills while conducting evidence examination and maintaining a chain of custody.
• Perform DNA isolation from various biological materials.
• Perform polymerase chain reaction (PCR) experiments with a Short Tandem Repeat (STR) multiplex.
• Compare the strengths and weaknesses of PCR experiments in the lab and in the field.
• Conduct quantification of DNA samples including an analysis of the presence of PCR inhibitors.
• Justify the importance of quality assurance and laboratory accreditation in the forensic laboratory.
• Perform appropriate contamination control techniques.
• Practice good laboratory safety including the use of personal protective equipment.
• Outline the process of fragment analysis for forensic DNA typing.
• Interpret single source and mixed DNA profiles including mixture deconvolution.
• Apply Bayes Theorem, the laws of probability, and Hardy Weinberg equilibrium to the statistical analysis of forensic DNA evidence.
• Perform various statistical calculations (Combined Probability of Inclusion, Random Match Probability and Likelihood Ratio) for DNA profiles.
• Follow standard operating procedures (SOPs) while working in a team environment.
• Demonstrate effective written and verbal communication skills to give effective presentations and write a comprehensive DNA report.

Effective as of Spring/Summer 2015

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