This six-module course will cover quantitative polymerase chain reaction (qPCR) amplification in the clinical setting. Early modules with cover nucleic acids from a clinical testing perspective and then focus will transition to reverse transcription (RT) qPCR for RNA viruses such as SARS-CoV-2 virus, causative agent of COVID-19. Correct interpretation of assay control results will be emphasized so that patient Cq results can be accurately interpreted and correct results reported.
Below is one offering of MLSC 1005 for the Spring/Summer 2022 term.
Start any time
- 6 weeks
- CRN 67540
Continuous Entry, Distance or Online
This is an online learning course. Start any time. You have 6 weeks from the date you register to complete this course.
Course outline TBD — see Learning Outcomes in the interim.
- Internet delivery format.
- Departmental approval needed
Program Coordinator: Dj Lidher Contact Info: firstname.lastname@example.org; work phone (604) 456-1257
Upon successful completion of this course, the student will be able to:
- describe how patient viral RNA samples are prepared and infection risks are mitigated
- describe strategies for minimizing DNA contamination risk in the PCR lab
- explain the principles of a multiplex RT-qPCR TaqMan assay
- interpret qPCR assay controls to prevent reporting potential false-negative/false-positive patient results
- correctly assess patient results referring to a lab's Interpretation and Reporting Job Aid
Effective as of Fall 2020
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Programs and courses are subject to change without notice.