Course Overview
A comprehensive introduction to diagnostic molecular techniques for constitutional genetic disease and cancer. This course will include foundational training in the basics of lab safety and human DNA preparation from various sources (blood, cell cultures, and solid tissue). In-class lectures, online resources and hands-on laboratory components of this course will also be focused on techniques such as the polymerase chain reaction (PCR) amplification, gel and capillary electrophoresis, and Sanger sequencing . Students will be introduced to specialty software used for the analysis, interpretation and validation of these assays. The theory behind Next Generation Sequencing (NGS) as a method for detection of pathogenic mutations will also be introduced.
Prerequisite(s)
- No prerequisites are required for this course.
Credits
10.5
- Not offered this term
- This course is not offered this term. Notify me to receive email notifications when the course opens for registration next term.
Learning Outcomes
By the end of this course, learners will be able to:
- Work safely in the laboratory while performing molecular diagnostic assays.
- Describe the transport of dangerous goods regulations relevant for Clinical Genetics Technology.
- Document and prepare specimens for analysis by performing several nucleic acid isolation methods and describing their respective advantages and limitations.
- Use techniques such as PCR amplification, restriction endonuclease digestion, and capillary electrophoresis to diagnose normal, affected, and carrier status in terms of genetic disease.
- Describe genetic diseases such as Hereditary Hemochromatosis and cancer in terms of mode of inheritance, population frequency, symptoms, and affected gene(s).
- Describe basic molecular diagnostic concepts for the constitutional and cancer lab, such as standard operating procedure, accessioning and Mendelian and neoplastic disorders.
- Explain the principles of Sanger and Next Generation sequencing methods.
- Perform and analyze data from Sanger sequencing reactions.
- Perform and document quality control on reagents and media.
- Describe gene mutations using Human Genome Variation Society nomenclature.
- Troubleshoot and solve common technical problems encountered while performing molecular techniques.
- Recognizes CCMG guidelines for specimen and record retention.
- Communicate effectively with the instructor and other students.
- Behave in a professional manner in lecture and lab sessions.
- Demonstrates inventory control skills.
Effective as of Fall 2021
Related Programs
Molecular Technology 1 (CLGT 5506) is offered as a part of the following programs:
School of Health Sciences
- Clinical Genetics Technology
Advanced Diploma Full-time
Programs and courses are subject to change without notice. Find out more about BCIT course cancellations.