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LC/MS and Applications CHEM 5005

Chemistry Course

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International Fees

International fees are typically three times the amount of domestic fees. Exact cost will be calculated upon completion of registration.

Course details

Covers the use of LC-MS in chemical analysis. The course starts with a general discussion of the theory of mass spectrometry as applied to the field of LC-MS. Types of LC-MS including LC-MSn are then compared. Subsequent sections of the course cover topics that relate to more advanced aspects of LC-MS including instrument components, modes of operation and the interpretation of LC-MS spectra. Other topics discussed include the optimization of quantitative and qualitative analyses using LC-MS and routine troubleshooting and maintenance of selected LC-MS instruments. Laboratory exercises provide practical training of the principles covered in the lectures.

Prerequisite(s)

Credits

3.0

Not offered this term
This course is not offered this term. Please check back next term or subscribe to receive notifications of future course offerings and other opportunities to learn more about this course and related programs.

Learning Outcomes

Upon successful completion, the student will be able to:

  • Demonstrate a thorough knowledge of the general theory of mass spectrometry where relevant to the field of liquid chromatography / mass spectrometry (LC/MS).
  • Discriminate the types of LC/MS according to their features and usage.
  • Compare LC/MS with LC/MSn.
  • Compare the various component options commonly found in LC/MS instruments.
    • Modes of ionization - atmospheric pressure chemical ionization (APCI) vs. atmospheric pressure electrospray (AP-ES).
    • Ion monitoring techniques - Positive vs. negative ion methods.
  • Explain the general steps involved in the operation of LC/MS systems.
  • Interpret LC/MS spectra.
  • Select operating parameters for a LC/MS separation by choosing appropriate mobile phase composition , column type, MS mode and MS parameters.
  • Compare LC/MS tuning procedures and analyse results from common tuning methods to plan LC/MS maintenance.
  • Optimise methods for qualitative and quantitative analysis using LC/MS.
  • Develop strategies for quantitative and qualitative analysis in LC/MS using common software packages.
  • Design procedures for method transfer between LC and LC/MS systems.
  • Plan and implement preventative maintenance procedures for use with LC/MS.
  • Select the correct logical steps involved in LC/MS troubleshooting.
  • Rank library generation strategies commonly used in LC/MS.
  • Illustrate the use of LC/MS in a range of applications including.
    • Determination of protein molecular weight.
    • Analysis of common macromolecules.
    • Pharmaceutical analysis.
    • Herbal analysis.

Effective as of Spring/Summer 2007

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