Course Overview
This course explores the structural and functional properties of proteins, and the diverse roles they play in living cells. Key principles of protein biochemistry will be applied to problems in the laboratory. Laboratory topics include the purification, quantification and characterization of proteins and enzymes. (UBC BIOT 311)
Prerequisite(s)
Credits
6.0
- Not offered this term
- This course is not offered this term. Notify me to receive email notifications when the course opens for registration next term.
Learning Outcomes
Upon successful completion, the student will be able to:
Lecture Learning Outcomes
- Discuss the basic principles governing biochemical interactions and the molecules involved.
- Illustrate, identify and describe in detail the structures and properties of the common amino acids
- Describe the relationship between protein structure and function.
- Describe common techniques used to explore protein structure and function.
- Manipulate 3-D computer models of proteins to visualize interactions
- Discuss the molecular basis for haemoglobin function.
- Discuss the molecular basis for hemoglobin function (allostery & cooperativity).
- Discuss the basic features and functions of enzymes.
- Discuss and analyze the kinetics of enzyme action.
- Discuss transition states and common mechanisms in enzyme catalysis.
- Describe the mechanisms of enzyme action of lysozyme and serine proteases.
- Discuss and analyze the kinetics of enzyme activity (Michaelis-Menten).
- Explain common mechanisms for the control of enzyme activity.
- Discuss protein folding, design and modeling of interactions.
- Describe the basic features of carbohydrates.
- Discuss the forces governing protein folding.
- Discuss protein misfolding and aggregation in neurodegenerative diseases.
Laboratory Learning Outcomes
- Quantify and characterize proteins using the Bradford assay and UV spectrometry.
- Isolate, purify and analyze proteins using the following techniques:
- Sonication and detergent lysis
- Ultra filtration
- Ion exchange and size exclusion chromatography
- SDS-PAGE and 2D-PAGE
- Salt fractionation
- Analyze and characterize enzymes using:
- Enzyme activity assays
- Vmax & KM determinations
- Specific activity determinations
- Perform basic bioinformatics of proteins by using online databases to search for functional information based on protein sequence and other information.
- Analyze raw data and write effective data reports.
Effective as of Fall 2021
Related Programs
Biochemistry 1 (BIOT 5240) is offered as a part of the following programs:
- Indicates programs accepting international students.
- Indicates programs with a co-op option.
- Indicates programs eligible for students to apply for Post-graduation Work Permit (PGWP).
School of Health Sciences
- Honours in Biotechnology
Bachelor of Science Full-time
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