British Columbia Institute of Technologywww.bcit.ca/study/courses/chem5005

BCIT

CHEM 5005 - LC/MS and Applications

Chemistry Part-time Studies Course

School of Computing and Academic Studies

Course Details

Covers the use of LC-MS in chemical analysis. The course starts with a general discussion of the theory of mass spectrometry as applied to the field of LC-MS. Types of LC-MS including LC-MSn are then compared. Subsequent sections of the course cover topics that relate to more advanced aspects of LC-MS including instrument components, modes of operation and the interpretation of LC-MS spectra. Other topics discussed include the optimization of quantitative and qualitative analyses using LC-MS and routine troubleshooting and maintenance of selected LC-MS instruments. Laboratory exercises provide practical training of the principles covered in the lectures.

Prerequisite(s)

Extensive HPLC knowledge or CHEM 5002.

Credits

3.0

This course isn't currently offered through BCIT Part-time Studies. Please check back next term or subscribe to receive email updates.

Learning Outcomes

Upon successful completion, the student will be able to:

  • Demonstrate a thorough knowledge of the general theory of mass spectrometry where relevant to the field of liquid chromatography / mass spectrometry (LC/MS).
  • Discriminate the types of LC/MS according to their features and usage.
  • Compare LC/MS with LC/MSn.
  • Compare the various component options commonly found in LC/MS instruments.
    • Modes of ionization - atmospheric pressure chemical ionization (APCI) vs. atmospheric pressure electrospray (AP-ES).
    • Ion monitoring techniques - Positive vs. negative ion methods.
  • Explain the general steps involved in the operation of LC/MS systems.
  • Interpret LC/MS spectra.
  • Select operating parameters for a LC/MS separation by choosing appropriate mobile phase composition , column type, MS mode and MS parameters.
  • Compare LC/MS tuning procedures and analyse results from common tuning methods to plan LC/MS maintenance.
  • Optimise methods for qualitative and quantitative analysis using LC/MS.
  • Develop strategies for quantitative and qualitative analysis in LC/MS using common software packages.
  • Design procedures for method transfer between LC and LC/MS systems.
  • Plan and implement preventative maintenance procedures for use with LC/MS.
  • Select the correct logical steps involved in LC/MS troubleshooting.
  • Rank library generation strategies commonly used in LC/MS.
  • Illustrate the use of LC/MS in a range of applications including.
    • Determination of protein molecular weight.
    • Analysis of common macromolecules.
    • Pharmaceutical analysis.
    • Herbal analysis.

Effective as of Spring/Summer 2007

Subscribe

Interested in being notified about future offerings of CHEM 5005 - LC/MS and Applications? If so, fill out the information below and we'll notify you by email when courses for each new term are displayed here.

Contact Information

Suggest course days and times

If you are interested in taking this course on-campus (classroom delivery only) and would like to see it offered on specific days and/or at specific times, please indicate your preferences below. Day and time suggestions are not applicable to online courses.

The personal information on this form is collected under the authority of the College and Institute Act (RSBC 1996, Ch.52). BCIT will use this information to communicate with you regarding relevant BCIT programs, courses and services. This information is only used by authorized BCIT staff. Email communication sent to and from BCIT is routed through the United States of America. If you have any questions about BCIT's collection and use of this information, please contact BCIT’s manager of Information Access and Privacy.

Programs and courses are subject to change without notice.

Find Courses

Payment Changes At BCIT

We've changed the way we accept payments.

Find out more

Course Navigation, Related Links & Tools

Registration News

Registration is now open for the Winter 2019 term.

Classroom Locations

Classroom locations are subject to last minute changes. Please check the Part-time Studies Classroom Locations listing at www.bcit.ca/rooms on the first day of any course you are registered for.

Part-time Studies Financial Aid

Will you be studying at BCIT part-time? Do you need assistance funding your studies? Find out if you qualify for Part-time Studies financial aid ›

© BCIT