Lesley Esford

lesford@my.bcit.ca

work experience

RESEARCH EXPERIENCE Ph.D. research (September 1993 - February 1999) Department of Microbiology and Immunology, University of British Columbia Thesis Title: The role of cell surface architecture in the HSV-1 lifecycle and in HA binding ability of CD44 Summary of PhD Research Variant L cell lines isolated on the basis of their resistance to herpes virus infection were used to characterize cellular components involved in viral entry and egress. Viral infectivity and the ability of virally infected cells to produce infectious virus and to form plaques were investigated using several techniques such as: plaque assays, indirect immunofluorescence, SDS-PAGE electrophoresis, immunoprecipitations, immunoblots, confocal and electron microscopy and flow cytometry.

M.Sc. research (September 1991 - August 1993)
Department of Biological Sciences, Brock University
Thesis title: The location and sequencing of the E3 and neighbouring regions in Bovine Adenovirus Type 2.

B.Sc. (Honours) research (Sept. 1988 - May 1989)
Department of Biological Sciences, Brock University
Thesis title: The mechanical properties of Gorgonians

TEACHING EXPERIENCE
British Columbia Institute of Technology - Instructor (November 1999 - present)

Jan 2002- present
Instructor and Program Head in the Joint BCIT/UBC Biotechnology Program
Responsible for all aspects of the courses. This includes curriculum development, the course outline, preparation of lectures, handouts, exam preparation, project assignments and evaluations and a total of 15 lecture hours per week.

Teaching assistant/Lab demonstrator in various courses (1991 - 1997)

ADMINISTRATIVE EXPERIENCE

British Columbia Institute of Technology - Program Head Biotechnology (September 2003 - present)
Schedule and facilitate departmental meetings, represent the department at school meetings, act as liaison with the biotechnology industry sector, manage department budget and administrative duties, facilitate student - faculty communication and conflict resolution, build awareness for Biotechnology with high school and university visits. I have represented the department and BCIT on international trade missions and conferences. I communicate regularly with BC Biotech as well as specific Biotechnology companies in the BC Lower Mainland to discuss co-operative education opportunities for students, employment opportunities, and to obtain feedback on our curriculum.

Committee Membership. I have been an active participant on the following committees:
Applied Research Committee, School of Health Sciences
School Quality Committee, School of Health Sciences
Sanofi-Aventis Biotech Challenge, Scientific Advisory Committee for Vancouver, Genome BC Education Advisory Committee

To support BCIT's mandate to increase research activities four-fold in the next five years, the Biotechnology department in collaboration with several other departments and research groups is trying to build capacity to conduct research aimed at improving the efficacy, quality and safety of Natural Health Products. I have taken a lead role with Paula Brown (Program Head of the Natural Health Products Research group) at establishing priorities, goals and securing funding.

British Columbia Institute of Technology - Program Head Basic Health Sciences (November 2000 - September 2001 )

Special projects: Acting program head and coordinator for Radiation Therapy

education

University of British Columbia, Vancouver, British Columbia, Canada
Ph.D. program (September 1993 - May 1999)
Thesis Supervisor: Dr. Frank Tufaro

Brock University, St. Catharines, Ontario, Canada
M.Sc. program (September 1991 - August 1993)
Thesis Supervisor: Dr. Yousef Haj-Ahmad

Brock University, St. Catharines, Ontario, Canada
Honours Business Administration (First class) (September 1988 - May 1991)

Brock University, St. Catharines, Ontario, Canada
B.Sc. (Hons), May 1988
"The Mechanical Properties of Gorgonians" (September 1987 - May 1988)
Thesis Supervisor: Dr. J.C. Lewis

publications

Peer-reviewed

1)L.E. Esford and J.C. Lewis. 1990. Stiffness of Caribbean gorgonians (coelenterata, octocorallia) and ca/mg content of their axes. Mar. Ecol. Prog. Ser. 67: 187-200.

2)K. Salmon, L.E. Esford and Y. Haj-Ahmad. 1993. Subcloning and restriction enzyme mapping of bovine adenovirus type 2. Intervirology 36: 72-78.

3)L.E. Esford and Y. Haj-Ahmad. 1994. Sequence analysis of the putative E3 region of bovine adenovirus type 2. Intervirology 37: 277-286.

4)Banfield B.W., Y. Leduc, L. Esford, R.J. Visalli, C.R. Brandt and F. Tufaro. 1995.
Evidence for an interaction of herpes simplex virus with chondroitin sulfate proteoglycans during infection. Virology 208: 531-539.

5)Banfield B.W., Y. Leduc, L. Esford, K. Schubert and F. Tufaro. 1995. Sequential isolation of proteoglycan synthesis mutants by using herpes simplex virus as a selective agent: evidence for a proteoglycan- independent virus entry pathway. Journal of Virology 69: 3290-3298.

6)Esford L.E., A. Maiti, S.A. Bader, F. Tufaro and P. Johnson. 1998. Analysis of CD44 interactions with hyaluronan in murine L cell fibroblasts deficient in glycosaminoglycan synthesis: a role for chondroitin sulfate. Journal of Cell Science 111: 1021-1029.

7)McCormick, C., Y. Leduc, D. Martindale, K. Mattison, L.E. Esford, A.P. Dyer and F. Tufaro. 1998. The putative tumour suppressor EXT1 alters the expression of cell-surface heparan sulfate. Nature Genetics 19: 158-161.

THESES

Esford, L.E. 1999. The role of the cell surface architecture in the HSV-1 lifecycle and in HA binding ability of CD44. Ph.D. thesis, University of British Columbia, Vancouver.

Esford, L.E. 1993: The locating and sequencing of the E3 and neighbouring regions in Bovine Adenovirus Type 2. M.Sc. thesis, Brock University, St. Catharines.

Esford, L.E. 1988: The mechanical properties of Gorgonians. B.Sc. (Honours) thesis, Brock University, St. Catharines.